Single-atom nanozymes with well-defined atomic constructions and digital coordination environments can successfully mimic the features of pure enzymes. Nevertheless, the pricey and complicated preparation processes have hindered additional exploration and utility of those single-atom nanozymes. On this research, we offered a synthesis approach for creating Fe–N central single-atom doped graphene quantum dot (FeN/GQDs) nanozymes utilizing a one-step solvothermal course of, the place particular person iron atoms kind sturdy bonds with graphene quantum dots by way of nitrogen coordination. In contrast to earlier research, this methodology considerably simplifies the synthesis situations for single-atom nanozymes, eliminating the necessity for top temperatures and using environmentally pleasant precursors derived from pineapple (ananas comosus) leaves. The ensuing FeN/GQDs exhibited peroxidase-like catalytic exercise and kinetics similar to that of pure enzymes, effectively changing H2O2 into hydroxyl radical species. Leveraging their wonderful peroxide-like exercise, FeN/GQDs nanozymes have been efficiently utilized to assemble a colorimetric biosensor system characterised by remarkably excessive sensitivity for glucose detection. This achievement demonstrated a promising strategy to designing single-atom nanozymes with each facile synthesis procedures and excessive catalytic exercise, providing potential purposes in wearable sensors and customized well being monitoring.
